Inhibition of the degradation of the precursor and of the mature β1 integrin subunit by different protein synthesis inhibitors and by ATP depletion
Abstract
A stabilization of both the precursor and the mature β1 integrin subunit was observed in metabolically labeled human skin fibroblasts and Molt-4 T lymphocytes upon addition of protein synthesis inhibitors or by ATP depletion. Differentia] effects of protein synthesis inhibitors are reported since the slow degradation of the mature β1 subunit was sensitive to cycloheximide but not to puromycin. We also show that the half-life of the mature subunit was not dependent on intracellular lysosomal degradation or on ubiquitination suggesting that VLA turn-over occurs at the cell surface and might involve proteins or proteases with short half-life.
- Publication:
-
FEBS Letters
- Pub Date:
- January 1993
- DOI:
- 10.1016/0014-5793(93)80171-P
- Bibcode:
- 1993FEBSL.327..207V
- Keywords:
-
- VLA degradation;
- Protein synthesis inhibitor;
- ATP depletion;
- Ubiquitin;
- Lysosome