Map-Based Cloning of a Gene Controlling Omega-3 Fatty Acid Desaturation in Arabidopsis
Abstract
A gene from the flowering plant Arabidopsis thaliana that encodes an omega-3 desaturase was cloned on the basis of the genetic map position of a mutation affecting membrane and storage lipid fatty acid composition. Yeast artificial chromosomes covering the genetic locus were identified and used to probe a seed complementary DNA library. A complementary DNA clone for the desaturase was identified and introduced into roots of both wild-type and mutant plants by Ti plasmid-mediated transformation. Transgenic tissues of both mutant and wild-type plants had significantly increased amounts of the fatty acid produced by this desaturase.
- Publication:
-
Science
- Pub Date:
- November 1992
- DOI:
- 10.1126/science.1455229
- Bibcode:
- 1992Sci...258.1353A