Yeast RNA polymerase II initiation factor e: isolation and identification as the functional counterpart of human transcription factor IIB.
Abstract
Yeast RNA polymerase II initiation factor e was purified to homogeneity and identified by biochemical criteria as the counterpart of human transcription factor IIB. Factor e was essential for initiation of transcription from yeast and mammalian promoters in a reconstituted yeast transcription system. Activity resided in a single polypeptide of approximately 41 kDa, identified by peptide sequence analysis as the product of the SUA7 gene. Factor e interacted specifically with RNA polymerase II, consistent with a proposed role in determining the start site of transcription.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- December 1992
- DOI:
- 10.1073/pnas.89.23.11292
- Bibcode:
- 1992PNAS...8911292T