Effects of experimental factors on the metabolic rate of t-lymphoma cells as measured by microcalorimetry

The heat production rate of T-lymphoma cells (CCRF-CEM) cultured in suspension was measured as a function of several experimental parameters. Changes in temperature and pH induced, as expected, significant variations In the heat production rate per cell. No such effects were observed to result from variations in cell concentration and stirring rate. The preparation method used here, with standardized treatment of the sample 24 h prior to measurement, produced samples in a reproducible condition with minor day-to-day variations.