Isolation and characterization of a lectin with exclusive specificity towards mannose from snowdrop (Galanthus nivalis) bulbs
Abstract
A lectin was isolated from snowdrop (Galanthus nivalis) bulbs by affinity chromatography on mannoseagarose. It is a tetrameric protein composed of 4 identical subunits of 13 kDa which are not held together by disulphide bridges. The G. nivalis agglutinin exhibits exclusive specificity towards mannose. Although it readily agglutinates rabbit erythrocytes it is completely inactive with human red blood cells.
- Publication:
-
FEBS Letters
- Pub Date:
- May 1987
- DOI:
- 10.1016/0014-5793(87)80129-1
- Bibcode:
- 1987FEBSL.215..140V
- Keywords:
-
- Characterization;
- Lectin;
- Mannose;
- Purification;
- (Galanthus nivalis);
- GNA;
- Galanthus nivalis agglutinin;
- SDS-PAGE;
- SDS-polyacrylamide gel electrophoresis;
- PBS;
- phosphate-buffered saline