Cloning and Complete Nucleotide Sequence of Human 5'-α -globin Gene
Abstract
We have cloned one of the two human alpha-globin genes and report its complete nucleotide sequence. The gene is 832 base pairs (bp) long from the 5'-cap site to the 3'-polyadenylylation site. The amino acid coding sequences are separated into three segments (exons) by two short (117 and 140 bp) intervening sequences. Highly conserved regions are identified in the 5'-flanking region, intron-exon junctions, and 3' noncoding regions that may have functional significance.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- December 1980
- DOI:
- 10.1073/pnas.77.12.7054
- Bibcode:
- 1980PNAS...77.7054L