The design and application of a vidicon rapid scanning spectrophotometer to stopped-flow kinetics
Abstract
A rapid scanning stopped-flow spectrophotometer was developed. The use of a silicon vidicon array detector enables reactions to be simultaneously monitored over a spectral region of approximately 260 nm. The instrument is controlled by a multi-microcomputer system. One microcomputer controls the vidicon spectrophotometer. The complete automation of the stopped-flow mixing system is handled by a second microcomputer. Another microcomputer handles interactions with the operator, mass data storage and communications with a laboratory minicomputer. A fourth microcomputer coordinates the activities of the other three. A new computer language called CONVERS-M was developed to operate in the multi-microcomputer system. The instrument was applied to an investigation of unusual reaction rates encountered in a kinetic method for determining the total protein content of human blood serum using the biuret reaction.
- Publication:
-
Ph.D. Thesis
- Pub Date:
- 1978
- Bibcode:
- 1978PhDT.......129C
- Keywords:
-
- Kinetics;
- Product Development;
- Scanners;
- Spectrophotometers;
- Vidicons;
- Design Analysis;
- Flow Measurement;
- Minicomputers;
- Numerical Control;
- Programming Languages;
- Silicon;
- Utilization;
- Instrumentation and Photography