Identification, Localization, and Role of Fibronectin in Cultured Bovine Endothelial Cells
Abstract
We have examined bovine aortic endothelial cell cultures for the presence of fibronectin, a high molecular weight cell-surface glycoprotein. Sparse cultures contain fibronectin only on dorsal cell surfaces at regions of cell-cell contact, as detected by immunofluorescence. In contrast, when the endothelial cells reached confluence as a highly contact-inhibited monolayer, fibronectin was detected in an extracellular matrix underneath the cell monolayer but not on top of the monolayer. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis of isolated extracellular matrix revealed that a predominant component of the matrix is a protein of approximately 2.3 × 105 molecular weight, which has been identified as fibronectin.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- July 1978
- DOI:
- Bibcode:
- 1978PNAS...75.3273B