HLA membrane antigens: sequencing by intrinsic radioactivity.
Abstract
Radiochemical sequence data are presented for the amino termini of mixed HLA antigens A1 and B8 isolated from a human lymphoblastoid cell line. Cells were labeled intrinsically in small-scale tissue culture with 14C-labeled amino acids and [14C]pyruvate. The specific activities obtained were sufficiently high and uniform to permit direct radiochemical sequencing of the antigens. HLA antigens were isolated by adsorption to a solid-phase anti-beta2-microglobulin immunoadsorbent followed by electrophoresis. The method should be generally applicable and useful for sequencing all parts of the molecule.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- December 1976
- DOI:
- 10.1073/pnas.73.12.4487
- Bibcode:
- 1976PNAS...73.4487B