Latent DNA of Epstein-Barr Virus: Separation from High-Molecular-Weight Cell DNA in a Neutral Glycerol Gradient
Abstract
Epstein-Barr virus DNA in virus non-productive cells was separated from high-molecular-weight cell DNA by sedimentation through a neutral glycerol gradient after gentle lysis of cells by Pronase and Sarkosyl. The isolated Epstein-Barr virus DNA had a density of 1.716-1.717 g/cm3 in CsCl equilibrium centrifugation, which is very close to the virus DNA density of 1.718 g/cm3. The results indicated that the majority of Epstein-Barr virus DNA in virus nonproductive cells is not covalently integrated into high-molecular-weight cell DNA.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- December 1974
- DOI:
- 10.1073/pnas.71.12.4658
- Bibcode:
- 1974PNAS...71.4658T