On the use of quinine sulfate as a fluorescence standard
Abstract
Using quinine sulfate in 0.1 N sulfuric acid as a standard during the spectroscopic study of purines ( 1), it was found that 2-aminopurine in neutral solution at 160°K has a fluorescence quantum yield which is about 2.5 times that of quinine sulfate at room temperature. Thus, the widely used value of 0.55 published for the absolute fluorescence quantum yield of quinine sulfate ( 2) is apparently too large. Presumably the value for quinine sulfate must be 0.4 or less; otherwise 2-aminopurine excited at this low temperature would have a fluorescence quantum yield in excess of unity and "two photon" emission seems unlikely.
- Publication:
-
Journal of Molecular Spectroscopy
- Pub Date:
- 1966
- DOI:
- 10.1016/0022-2852(66)90267-0
- Bibcode:
- 1966JMoSp..19..454D