Spectroscopy of Cells: the Use of a Reflecting Microscope and Spectrographic Recording
Abstract
THIS communication describes the use of a simple microspectroscopic method which permits the recording of the ultra-violet and the visible absorption characteristics of cells or portions of cells within a period of a few seconds. The apparatus consists of a light source which emits radiation throughout the ultra-violet and the visible spectrum, a microscope with a reflecting achromatic condenser and objective of numerical aperture 0.56 (designed by Mr. A. J. Kavanagh and made by the Scientific Instrument Division of the American Optical Company), and a small quartz spectrograph (Hilger).
- Publication:
-
Nature
- Pub Date:
- July 1950
- DOI:
- 10.1038/166026a0
- Bibcode:
- 1950Natur.166...26M