Identification of molecular mechanism in breast cancer cell induced by simulated microgravity after Random Positioning Machine exposure
Abstract
Simulated microgravity (SM) provides a unique environment to study how disease cells including cancers, respond to mechanical unloading. In this study, breast cancer cell MDA-MB-231 exposed to 48hrs of SM displayed detachment from growth surface, formation of multiple cell aggregates and irregular cell proliferation. Morphological characteristics of cancer cell cytoskeleton were significantly altered after SM. Gene expression levels that are relevant to 3 dimensions spheroid formation, cytoskeleton organization and extracellular matrix formation showed significant changes. Furthermore, it was observed that the breast cancers cell cycle was halted before the mitosis phase under SM. Overall, these results outline the significant role "gravity" plays in the form of mechanical stimuli in breast cancer cell's function and survival. This approach can lead to the development of future cancer therapeutics targeting specifically the mechanosensing pathways of breast cancers.
- Publication:
-
43rd COSPAR Scientific Assembly. Held 28 January - 4 February
- Pub Date:
- January 2021
- Bibcode:
- 2021cosp...43E2068W