Optimization of the Extraction Process of Anti-Vibrio parahaemolyticus Active Substances from Coptidis Rhizoma (Huanglian)
Abstract
The purpose of this study is to optimize the extraction process of the anti-Vibrio parahaemolyticus active substances from Coptidis rhizoma (Huanglian). Single factor experiment, Box-Behnken experimental design combined with response surface methodology were used to optimize the extraction process. The obtained optimal extraction conditions followed as: extraction solvent is 100% methanol, extraction temperature of 86 °Q liquid/material ratio of 21 mL/g’ extraction time of 116 min. The actual value of the inhibition zone of the extract can reach 15.62±0.16 mm, which is not significantly different from the predicted value of 15.12 mm. This indicates that the above method is feasible to optimize the extraction process of the anti-V. parahaemolyticus active substances from Coptidis rhizoma.
- Publication:
-
Journal of Physics Conference Series
- Pub Date:
- September 2020
- DOI:
- 10.1088/1742-6596/1637/1/012108
- Bibcode:
- 2020JPhCS1637a2108W