Regulation of type II restriction-modification system Esp1396I
Abstract
Fluorescence microscopy is a powerful tool which enables to observe a large number of processes at the level of single cells. A protein of interest could be selectively labeled using fluorescent proteins, thereby allowing direct observation of its accumulation in living cells. This work is devoted to study a restriction-modification system which is a protective system of bacteria against foreign DNA. The action of such a system is based on DNA degradation by an enzyme — restriction endonuclease — and is strongly regulated to prevent host cell DNA damaging. In current study, the restriction-modification system contains controller protein, which regulates amount of restriction-modification system enzymes. Changes in amount of restriction-modification system proteins may cause a change in protection level of bacteria against viruses. We use fluorescence microscopy for direct observation of restriction-modification protein levels and their changes depending on the amount of controller protein in bacterial cells.
- Publication:
-
Journal of Physics Conference Series
- Pub Date:
- November 2019
- DOI:
- 10.1088/1742-6596/1400/3/033024
- Bibcode:
- 2019JPhCS1400c3024A