Atmospheric Aging Effects on the Toxicity of Naphthalene Secondary Organic Aerosol
Abstract
Epidemiological studies have shown that exposure to ambient particles is associated with numerous adverse health outcomes. However, the dynamic toxicity of aerosol and the potential pathophysiological mechanisms during atmospheric processing is poorly understood. Being emitted from a variety of sources, such as wood burning, vehicle engine, petroleum processing and cigarette smoke, naphthalene is the most ubiquitous polycyclic aromatic hydrocarbon (PAH) in ambient. Using secondary organic aerosol derived from naphthalene photooxidation (NSOA) as a model system, comprehensive chemical characterization was performed using advanced mass spectrometry for SOA experienced various aging conditions. Both acellular and cellular assays were applied in investigating the dynamic toxicity of NSOA evolved throughout atmospheric aging. As the least ambiguous method for free radical measurements, electron paramagnetic resonance (EPR) was used to profile the oxidative potential (OP) of NSOA by measuring the reactive oxygen species (ROS) directly. Measured acellular OP presents a consistent trend with the cytotoxicity manifested by the BEAS-2B cells exposed to NSOA. Besides the redox-active quinone content that can explain the toxicity of NSOA, mass spectrometry-based proteomics coupled with activity-based protein profiling (ABPP) was used to study the impact of NSOA on protein expression during atmospheric processing. Confirmed by the molecular cysteine probe, proteome-wide effects show that the short-lived carbonyls in NSOA evolved along with photochemical aging might be the major factor for its toxicity in vitro.
- Publication:
-
AGU Fall Meeting Abstracts
- Pub Date:
- December 2019
- Bibcode:
- 2019AGUFMGH41A..01W
- Keywords:
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- 0305 Aerosols and particles;
- ATMOSPHERIC COMPOSITION AND STRUCTURE;
- 0345 Pollution: urban and regional;
- ATMOSPHERIC COMPOSITION AND STRUCTURE;
- 0394 Instruments and techniques;
- ATMOSPHERIC COMPOSITION AND STRUCTURE;
- 0240 Public health;
- GEOHEALTH