Temperature at the focal point of optical trapping beam: evaluation using fluorescence correlation spectroscopy
Abstract
Fluorescence correlation spectroscopy was applied to the evaluation of the local heating at the focal spot of nearinfrared laser for optical trapping. Based on the translational diffusion coefficient of probe dyes at the focal spot in solution, the relation between temperature rise and incident laser power, ΔT/ΔP, were determined for water, ethylene glycol, 1-pentanol, 1-hexanol, 1-octanol, 1-nonanol, and 1-decanol. The value of ΔT/ΔP linearly increased with a/l (a and l is the absorption coefficient and thermal conductivity of solvent, respectively) as predicted by a simple theoretical model.
- Publication:
-
Optical Manipulation Conference
- Pub Date:
- April 2018
- DOI:
- 10.1117/12.2319650
- Bibcode:
- 2018SPIE10712E..1YS