A benzothiazole-based fluorescent probe for hypochlorous acid detection and imaging in living cells
Abstract
A benzothiazole-based turn-on fluorescent probe with a large Stokes shift (190 nm) has been developed for hypochlorous acid detection. The probe displays prompt fluorescence response for HClO with excellent selectivity over other reactive oxygen species as well as a low detection limit of 0.08 μM. The sensing mechanism involves the HClO-induced specific oxidation of oxime moiety of the probe to nitrile oxide, which was confirmed by HPLC-MS technique. Furthermore, imaging studies demonstrated that the probe is cell permeable and can be applied to detect HClO in living cells.
- Publication:
-
Spectrochimica Acta Part A: Molecular Spectroscopy
- Pub Date:
- June 2018
- DOI:
- 10.1016/j.saa.2018.03.055
- Bibcode:
- 2018AcSpA.199..189N
- Keywords:
-
- Fluorescent probe;
- Large Stokes shift;
- Hypochlorous acid;
- Cell imaging;
- Benzothiazole