Measuring soil organic matter turn over and carbon stabilisation in pasture soils using 13C enrichment methodology.
Abstract
Carbon storage in soil is a balance between photosynthesis and respiration, however, not all C compounds decompose equally in soil. Soil C consists of several fractions of C ranging from, accessible C (rapidly cycling) to stored or protected C (slow cycling). The key to increasing C storage is through the transfer of soil C from this accessible fraction, where it can be easily lost through microbial degradation, into the more stable fraction. With the increasing use of isotope enrichment techniques, 13C may be used to trace the movement of newly incorporated carbon in soil and examine how land management practises affect carbon storage. A laboratory method was developed to rapidly analyse soil respired CO2 for δ13C to determine the temperature sensitivity of newly incorporated 13C enriched carbon. A Horotiu silt loam (2 mm sieved, 60% MWHC) was mixed with 13C enriched ryegrass/clover plant matter in Hungate tubes and incubated for 5 hours at 20 temperatures( 4 - 50 °C) using a temperature gradient method (Robinson J. M., et al, (2017) Biogeochemistry, 13, 101-112). The respired CO2 was analysed using a modified Los Gatos, Off-axis ICOS carbon dioxide analyser. This method was able to analyse the δ13C signature of respired CO2 as long as a minimum concentration of CO2 was produced per tube. Further analysis used a two-component mixing model to separate the CO2 into source components to determine the contribution of added C and soil to total respiration. Preliminary data showed the decomposition of the two sources of C were both temperature dependant. Overall this method is a relatively quick and easy way to analyse δ13C of respired soil CO2 samples, and will allow for the testing of the effects of multiple variables on the decomposition of carbon fractions in future use.
- Publication:
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AGU Fall Meeting Abstracts
- Pub Date:
- December 2017
- Bibcode:
- 2017AGUFM.B51D1825R
- Keywords:
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- 0412 Biogeochemical kinetics and reaction modeling;
- BIOGEOSCIENCES;
- 0414 Biogeochemical cycles;
- processes;
- and modeling;
- BIOGEOSCIENCES;
- 1615 Biogeochemical cycles;
- processes;
- and modeling;
- GLOBAL CHANGE;
- 1631 Land/atmosphere interactions;
- GLOBAL CHANGE