Next Generation Image-Based Phenotyping of Root System Architecture
Abstract
The development of the Plant Root Imaging and Data Acquisition (PRIDA) hardware/software system enables researchers to collect digital images, along with all the relevant experimental details, of a range of hydroponically grown agricultural crop roots for 2D and 3D trait analysis. Previous efforts of image-based root phenotyping focused on young cereals, such as rice; however, there is a growing need to measure both older and larger root systems, such as those of maize and sorghum, to improve our understanding of the underlying genetics that control favorable rooting traits for plant breeding programs to combat the agricultural risks presented by climate change. Therefore, a larger imaging apparatus has been prototyped for capturing 3D root architecture with an adaptive control system and innovative plant root growth media that retains three-dimensional root architectural features. New publicly available multi-platform software has been released with considerations for both high throughput (e.g., 3D imaging of a single root system in under ten minutes) and high portability (e.g., support for the Raspberry Pi computer). The software features unified data collection, management, exploration and preservation for continued trait and genetics analysis of root system architecture. The new system makes data acquisition efficient and includes features that address the needs of researchers and technicians, such as reduced imaging time, semi-automated camera calibration with uncertainty characterization, and safe storage of the critical experimental data.
- Publication:
-
AGU Fall Meeting Abstracts
- Pub Date:
- December 2016
- Bibcode:
- 2016AGUFM.B54D..06D
- Keywords:
-
- 0428 Carbon cycling;
- BIOGEOSCIENCESDE: 0452 Instruments and techniques;
- BIOGEOSCIENCESDE: 0476 Plant ecology;
- BIOGEOSCIENCESDE: 1865 Soils;
- HYDROLOGY