Soil nitrogen cycling and availability are linked to ammonia oxidizer abundance across a tropical mean annual temperature gradient
Abstract
Interactions among environmental variables can obfuscate the primary drivers linking soil microbial community function to ecosystem biogeochemistry. These connections are important to understand in order to predict ecosystem responses to global climate change. In particular, the role of mean annual temperature (MAT) in regulating carbon (C) and nitrogen (N) cycling via microbial communities remains unclear. To study these dynamics in situ, we used a a natural elevation gradient of tropical wet montane forest on Mauna Kea, Hawai'i with established permanent plots. Across the gradient, environmental variables besides MAT remain constant. We studied the abundance and activity of the amoA gene, which regulates the rate-limiting step of nitrification, in ammonia oxidizing archaea (AOA) and bacteria (AOB) with relation to N availability and cycling across increasing MAT. Our results show that the abundance of amoA is positively correlated with MAT (p<0.05; r2=0.34) and that MAT and amoA abundance are the primary predictors of nitrate (NO3-) bioavailability (p<0.05). We also found that the relative expression of amoA (cDNA/DNA) is not correlated with MAT or potential net nitrification rate. Our results indicate the direct role of MAT in ammonia oxidizer community structure and demonstrate feedbacks to nutrient availability in forest systems. These findings suggest that forest primary production and carbon cycling may be affected by AOA and AOB responses to rising MAT.
- Publication:
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AGU Fall Meeting Abstracts
- Pub Date:
- December 2016
- Bibcode:
- 2016AGUFM.B13D0637P
- Keywords:
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- 0414 Biogeochemical cycles;
- processes;
- and modeling;
- BIOGEOSCIENCESDE: 0439 Ecosystems;
- structure and dynamics;
- BIOGEOSCIENCESDE: 0465 Microbiology: ecology;
- physiology and genomics;
- BIOGEOSCIENCESDE: 0470 Nutrients and nutrient cycling;
- BIOGEOSCIENCES