Preparation, characterization and pharmacokinetics of fluorescence labeled propylene glycol alginate sodium sulfate
Abstract
A rapid and sensitive fluorescence labeling method was developed and validated for the microanalysis of a sulfated polysaccharide drug,namely propylene glycol alginate sodium sulfate (PSS), in rat plasma. Fluorescein isothiocyanate (FITC) was selected to label PSS, and 1, 6-diaminohexane was used to link PSS and FITC in order to prepare FITC-labeled PSS (F-PSS) through a reductive amination reaction. F-PSS was identified by UV-Vis, FT-IR and 1H-NMR spectrum. The cell stability and cytotoxicity of F-PSS were tested in Madin-Darby canine kidney (MDCK) cells. The results indicated that the labeling efficiency of F-PSS was 0.522% ± 0.0248% and the absolute bioavailability was 8.39%. F-PSS was stable in MDCK cells without obvious cytotoxicity. The method was sensitive and reliable; it showed a good linearity, precision, recovery and stability. The FITC labeling method can be applied to investigating the absorption and metabolism of PSS and other polysaccharides in biological samples.
- Publication:
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Journal of Ocean University of China
- Pub Date:
- August 2014
- DOI:
- 10.1007/s11802-014-2273-6
- Bibcode:
- 2014JOUC...13..683L
- Keywords:
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- propylene glycol alginate sodium sulfate;
- fluorescence labeling;
- fluorescein isothiocyanate;
- pharmacokinetics