The Role of Snow Thickness over Arctic Winter Sea Ice in the Survival and Dispersal of Brine-Derived Microbes
Abstract
The brines of polar winter sea ice are inhabited by significant densities of microbes (Bacteria and Archaea) that experience a range of extreme conditions depending on location in, and age of, the ice. Newly formed sea ice in winter expels microbes (and organic exudates) onto the surface of the ice, where they can be wicked into frost flowers or into freshly deposited snow, resulting in populations at the ice-air and air-snow interfaces characterized by even more extreme conditions. The influence of snow thickness over the ice on the fate of these microbes, and their potential for dispersal or mediation of exchanges with other components of the ice-snow system, is not well known. Examination of in situ temperature data from the Mass Balance Observatory (MBO) offshore of Barrow, Alaska, during the winter of 2011 allowed recognition of an hierarchy of fluctuation regimes in temperature and (by calculation) brine salinity, where the most stable conditions were encountered within the sea ice and the least stable highest in the snow cover, where temperature fluctuations were significantly more energetic as determined by an analysis of power spectral density. A prior analysis of snow thickness near the MBO had already revealed significant ablation events, potentially associated with bacterial mortality, that would have exposed the saline (microbe-rich) snow layer to wind-based dispersal. To better understand the survival of marine bacteria under these dynamic and extreme conditions, we conducted laboratory experiments with Arctic bacterial isolates, subjecting them to simulations of the freezing regimes documented at the MBS. The impact of the fluctuation regime was shown to be species-specific, with the organism of narrower temperature and salinity growth ranges suffering 30-50% mortality (which could be partially relieved by providing protection against salt-shock). This isolate, the psychrophilic marine bacterium Colwellia psychrerythraea strain 34H (temperature range of -12 to 18°C, salinity range of 20 to 50), was originally isolated from Arctic marine sediments. The other isolate, the psychrotolerant and extremely halophilic bacterium Psychrobacter sp. strain 7E (temperature range of -1 [possibly lower] to 25°C, salinity range of 32 to 125), not only survived the most extreme conditions but demonstrated a potentially effective dispersal strategy of cell fragmentation and miniaturization (resulting in higher cell numbers). This extremophile was isolated from upper winter sea-ice brine in the Beaufort Sea. Bacterial survival and dispersal from sea-ice brines in Arctic winter thus appears to depend on the nature of the organisms involved and on the thickness of snow cover, which determines how dynamic and extreme are the exposure conditions. The observed species-specific reactions to extreme and fluctuating conditions may help to explain the different structures of microbial communities inhabiting the range of environments defined by the ice-snow system and provide model organisms and research directions for future work to evaluate potential activity or exchanges with other components of the system.
- Publication:
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AGU Fall Meeting Abstracts
- Pub Date:
- December 2013
- Bibcode:
- 2013AGUFM.C31A0625D
- Keywords:
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- 0750 CRYOSPHERE Sea ice;
- 0465 BIOGEOSCIENCES Microbiology: ecology;
- physiology and genomics;
- 4840 OCEANOGRAPHY: BIOLOGICAL AND CHEMICAL Microbiology and microbial ecology