Functional Analysis of the Genes of Yeast Chromosome V by Genetic Footprinting
Abstract
Genetic footprinting was used to assess the phenotypic effects of Ty1 transposon insertions in 268 predicted genes of chromosome V of Saccharomyces cerevisiae. When seven selection protocols were used, Ty1 insertions in more than half the genes tested (157 of 268) were found to result in a detectable reduction in fitness. Results could not be obtained for fewer than 3 percent of the genes tested (7 of 268). Previously known mutant phenotypes were confirmed, and, for about 30 percent of the genes, new mutant phenotypes were identified.
- Publication:
-
Science
- Pub Date:
- December 1996
- DOI:
- 10.1126/science.274.5295.2069
- Bibcode:
- 1996Sci...274.2069S