Dissecting the energetic architecture within an RNA tertiary structural motif via high-throughput thermodynamic measurements
Abstract
Properly folded RNAs perform essential biological processes. Such RNAs often contain tertiary contact "motifs" whose structural and energetic properties are conserved throughout different RNAs. This study delves into the energetic architecture of one common RNA motif, the 11-nucleotide receptor. As deep studies into the energetic properties of this and other RNA motifs require thermodynamic measurements for many sequence variants, we used a quantitative, high-throughput method to obtain binding free energies for all single and double mutants of the motif. In characterizing the energetic architecture of this motif, our study uncovered patterns of energetic coupling within the motif and identified rare variants with unexpected properties.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- March 2023
- DOI:
- 10.1073/pnas.2220485120
- Bibcode:
- 2023PNAS..12020485S