Viable offspring derived from single unfertilized mammalian oocytes
Abstract
In mammals, parthenogenesis is limited because of problems arising from genomic imprinting. Here, we report live mammalian offspring derived from single unfertilized eggs. This was achieved by the targeted DNA methylation rewriting of seven imprinting control regions. By designing guide RNAs with protospacer adjacent motif (PAM) sequences matching one allele but not the other, dCas9-Dnmt3a or dCpf1-Tet1 enables targeted DNA methylation editing in an allele-specific manner. The success of parthenogenesis in mammals opens many opportunities in agriculture, research, and medicine.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- March 2022
- DOI:
- 10.1073/pnas.2115248119
- Bibcode:
- 2022PNAS..11915248W