DefocusTracker: A modular toolbox for defocusing-based, single-camera, 3D particle tracking
Abstract
The need for single-camera 3D particle tracking methods is growing, among others, due to the increasing focus in biomedical research often relying on single-plane microscopy imaging. Defocusing-based methods are ideal for a wide-spread use as they rely on basic microscopy imaging rather than requiring additional non-standard optics. However, a wide-spread use has been limited by the lack of accessible and easy-to-use software. DefocusTracker is an open-source toolbox based on the universal principles of General Defocusing Particle Tracking (GDPT) relying solely on a reference look-up table and image recognition to connect a particle's image and its respective out-of-plane depth coordinate. The toolbox is built in a modular fashion, allowing for easy addition of new image recognition methods, while maintaining the same workflow and external user interface. DefocusTracker is implemented in MATLAB, while a parallel implementation in Python is in the preparation.
- Publication:
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arXiv e-prints
- Pub Date:
- February 2021
- DOI:
- 10.48550/arXiv.2102.03056
- arXiv:
- arXiv:2102.03056
- Bibcode:
- 2021arXiv210203056B
- Keywords:
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- Physics - Fluid Dynamics
- E-Print:
- 11 pages, 3 figures, submitted for Journal of Open Research Software (JORS)