PolyWAG (Water aquired Genomics) Opensource 24-filter eDNA Water Sampler - Robotic DNA Collection with High Performance Sample Isolation and Preservation
Abstract
Organisms leave DNA in the environment as they pass through and its detection is crucial in some studies. Environmental DNA collection in the current market is done manually, where each sample needs to be collected by hand and individually filtered. Alternatively, commercial automated options are expensive and usually require an operator. The eDNA sampler we have developed is a water sampling device that collects DNA samples via 24, 45mm filter holders and provides a non-invasive, safe, and autonomous means of DNA collection. Our features include 24 inline replaceable and reusable filter holder units. The sampler has sleep mode enabled (130uA) along with 80mL/min flow rate. The design is openly published, modular and is being constantly tested to help us optimize our firmware to give us the best results. To control the sampler, we have a browser app for real-time monitoring, scheduling tasks, and manual operation as well as data logging for time, pressure, flow, filtered volume, and water temperature. We use a 9-step sampling sequence that helps reduce cross contamination significantly as verified by lab tests of samples with quantitative PCR. The sampler is also equipped with a programmable safety shut off that will terminate the sampling sequence in case of high pressures, flow rates or any parameter of the users choosing. As a result, we have a machine that can be deployed for an extended period of time, while being completely autonomous in terms of sampling and conserving battery life to help extend deployment periods even further. Our latest sampler costs around $5000 each.
- Publication:
-
AGU Fall Meeting Abstracts
- Pub Date:
- December 2021
- Bibcode:
- 2021AGUFM.H45T1432W