In this work, we present a new microspectrometry FTIR-based biosensor for the analysis of sugar based solutions using various solvent and various types of sample handling support (SHS). We used methanol as solvent because it does not interact with infrared (IR) beam. In addition, it ensures a rapid evaporation in order to form a thin layer of targeted molecules on the conventional SHS. The later consists of a total reflective mirror (TRM). Because of methanol toxicity, we developed a new setup for aqueous samples analysis based on accelerated solvent evaporation. The achieved limit of detection (LOD) using the developed biosensor was 3 mM for both, Glucose and Fructose molecules.