Collective effects of XMAP215, EB1, CLASP2, and MCAK lead to robust microtubule treadmilling

Treadmilling is a complex behavior of active polymers characterized by polymerization at one polymer end and simultaneous depolymerization at the other end. Treadmilling is an essential feature of cytoskeletal filaments driving actin-based cell motility, bacterial cell division and transport, and reorganization of microtubule arrays in plants. Although microtubule treadmilling occurs in many cellular contexts, how cells coordinate growth at microtubule plus ends and shrinkage at microtubule minus ends to achieve treadmilling is not understood. Here, we employ predictive computational modeling and a multiprotein in vitro assay to reconstitute cellular-like microtubule treadmilling. Our work provides a deeper understanding of how active polymer systems can be tuned to give rise to robust yet dynamic cytoskeletal architectures.