Isolation and characterisation of Salmonella sp. in captive crested geckos (Correlophus ciliatus)
Abstract
Salmonella is a gram-negative faecal coliform bacteria from the family enterobacteriaceae , affecting both humans and animal species. Salmonella is often present in reptiles including the crested gecko ( Correlophus ciliatus ) . While most reptiles do not present any symptoms of salmonellosis and typically appear healthy, the endemic nature of Salmonella may lead to infection in humans through direct contact of reptiles or both indirect and direct contact of the infected faeces. In humans, Salmonella may cause symptoms including nausea, vomiting, diarrhoea , fever and even death. Since the crested gecko is a common household pet in Hong Kong, this study examines its potential as a reservoir for Salmonella sp. in order to determine whether it might pose a risk to human health.
Faecal samples of captive-bred household pet crested geckos were resuspended in saline and serial-diluted and extracted bacteria grown on CAS+ (CHROMagar Salmonella Plus), CAS (CHROMagar Salmonella), and CAO (CHROMagar Orientation) agar plates in order to identify Salmonella colonies. Three Salmonella isolates were chosen and streaked for eight generations on LB plates before DNA was extracted using the Invitrogen PureLink Genomic DNA Mini Kit.- Publication:
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AGU Fall Meeting Abstracts
- Pub Date:
- December 2019
- Bibcode:
- 2019AGUFMED41B1085B
- Keywords:
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- 0805 Elementary and secondary education;
- EDUCATION