Structural basis for murine norovirus engagement of bile acids and the CD300lf receptor
Abstract
The mechanisms of norovirus capsid interactions with host receptors and the mechanisms by which soluble cofactors augment norovirus infection are not understood. We recently identified CD300lf as a cell surface receptor for murine norovirus (MNoV) and observed that a small molecule cofactor was critical for efficient binding of virus to CD300lf. Herein we identify the bile acid GCDCA as a cofactor enhancing MNoV infection and provide a biophysical characterization of the capsid-receptor and capsid-cofactor interactions, thereby providing a structure-based understanding of how noroviruses initiate cellular infection. This work has important implications for the design of norovirus therapeutics.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- September 2018
- DOI:
- 10.1073/pnas.1805797115
- Bibcode:
- 2018PNAS..115E9201N