MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution
Abstract
Popular localization of single molecules through calculating the centroid of the diffraction pattern produced by molecular fluorescence on a camera is typically limited to spatiotemporal resolutions of >10 nm per >10 milliseconds. By requiring at least 10-100 times fewer detected photons and being free of bias due to molecular orientation, the localization concept called MINFLUX propels molecular tracking to the hitherto-unachievable regime of single-digit nanometer precision within substantially less than a millisecond. Our experiments herald the feasibility to detect molecular interactions and conformational changes at microsecond timescales.
- Publication:
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Proceedings of the National Academy of Science
- Pub Date:
- June 2018
- DOI:
- 10.1073/pnas.1801672115
- Bibcode:
- 2018PNAS..115.6117E