The aim of this work was to investigate the physiochemical and mechanical properties of gellan gum incorporating norfloxacin (GG-NOR) biofilm at different concentration of NOR. Four different concentration of NOR (0.01%, 0.1%, 0.5%, and 1% (w/w)) were used to produce GG-NOR biofilms. The biofilms was successfully produced with good shape and were transparent at ∼95%. The addition of norfloxacin was found to increase the toughness and elongation-at-break values of biofilm to 0.52 J g-1 and 3.8 % at higher concentration of NOR. On top of that, the thermal stability of biofilm also was increased when the concentration of norfloxacin was increased attributed to the interaction between gellan gum and norfloxacin as proved by FTIR analysis.