A virtual laboratory known as VICH-LAB (Virtual Chemistry Laboratory) has been developed in hybrid learning. This VICH-LAB was used in electrochemistry classes involving much practice work with various tools and materials. VICH-LAB contains a MSDS (Material Safety Data Sheet), tools introduction, interactive practicum, and assignments. The aim of this study was to measure self-efficacy in the use of VICH-LAB. The design of this research was quasi-experimental with a post-test design. Differences among 3 classes with different treatments was observed. The first class used a wet laboratory, the second class used a virtual laboratory integrated hybrid learning approach, and a third class used both of them. All three classes took place during the same academic term and had the same teachers. The variable measured in the three classes was self-efficacy, measured with a questionnaire consisting of 22 items. Self-efficacy scores were significantly different between the class using a wet laboratory and the class that was using the wet laboratory and VICH-LAB as a supplement.