Partitioning of evapotranspiration in the highest alpine meadow zones through in-situ laser-based chamber and dual water stable isotopes approaches

Understanding plant functionality within the water cycles of grassland ecosystems is crucial for obtaining both regional water balance and plant adaptability in the context of ongoing climate change. The transpiration to evapotranspiration ratio (T/ET) is an indicator of plant's contribution to ecosystem water cycle. In this study, we used high-frequency laser spectroscopy (Picarro L2130-i), three custom-built chambers, and eddy covariance techniques, to constrain the role played by plants in evapotranspiration over an alpine meadow ecosystem in the central Tibetan Plateau (TP). Three different sizes of chambers are used to direct measure the isotopic compositions in evapotranspiration (δ_ET), evaporation (δ_E) and transpiration (δ_T). The consistent T/ET between δ¹⁸O and δD manifests that chamber and dual isotope tracers are robust methods to estimate T/ET in alpine meadow zone. The results from this study indicate that plants play an important role in the water cycles of alpine meadow ecosystems despite the sparse distribution of plant cover. We also synthesized the published T/ET data over the entire TP region, and found a good spatial relation between T/ET and leaf area index (LAI). Moreover, soil water content played some role in controlling T/ET beyond the LAI in arid/semiarid regions such as the TP. More than half of the TP is covered by grassland, but its low biomass and shallow rooting depth make it very vulnerable to climate change variables such as air temperature warming and variations in precipitation. Given the crucial role played by plants in an ecosystem's water cycle, any variations in grassland cover are likely to exert a critical impact on the regional hydrological cycle, and even the regional climate.