Large-scale identification of coevolution signals across homo-oligomeric protein interfaces by direct coupling analysis
Abstract
Protein-protein interactions are important to all facets of life, but their experimental and computational characterization is arduous and frequently of uncertain outcome. The current study demonstrates both the power and limitation to study protein interactions by utilizing sophisticated statistical inference technology to derive protein contacts from available sequence databases, more precisely from the coevolution between residues, that are in contact across the interaction interface of two proteins. By studying homo-oligomeric protein interactions, the current study expands from anecdotal evidence of the performance of this technology to systematic evidence of its value across close to 2,000 interacting protein families.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- March 2017
- DOI:
- 10.1073/pnas.1615068114
- arXiv:
- arXiv:1703.01246
- Bibcode:
- 2017PNAS..114E2662U
- Keywords:
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- homo-oligomers;
- coevolution;
- direct coupling analysis;
- protein–protein interactions;
- big data analysis;
- Quantitative Biology - Biomolecules
- E-Print:
- 18 pages, 6 figures, to appears in PNAS