Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
Abstract
Biocompatible microfluidic double water-in-oil-in-water emulsion (MDE) enables in-droplet cultivation of different living species. The combination of droplet-generating machinery with FACS followed by next-generation sequencing and liquid chromatography-mass spectrometry analysis of the secretomes of encapsulated organisms yielded detailed genotype/phenotype descriptions. The MDE-FACS platform we developed enabled highly sensitive single-cell selection of predesigned activity and exploration of pairwise interactions between target and effector cells without interference from other microbiota species.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- March 2017
- DOI:
- 10.1073/pnas.1621226114
- Bibcode:
- 2017PNAS..114.2550T