Undergraduate Collaborative Research: Distribution of Plant Wax Biomarkers in Miocene-age Sediments from the Bengal Fan (IODP Exp 354)

Our research aims to understand past climatic variability in the monsoon-influenced Ganges-Brahmaputra catchment as recorded by plant wax molecules exported and sequestered in the sediments of the Bengal Fan. Samples from the late Miocene were selected from cores retrieved by the IODP (International Ocean Discovery Program) Expedition 354 that recently drilled the central Bengal Fan along a transect at 8°N. Fan sedimentation includes sand, silt, and clay mostly derived from the Himalayan range via turbiditic transport within the Bengal fan. Sedimentation is highly episodic in the fan, but a transect of drilled sites provides a record of terrigenous sediment exported and buried over the last 20 million years. A team of researchers at the University of Southern California worked to collectively process 468 samples for compound specific biomarker identification and quantification. The samples derive from Site U1451 and U1455 ranging from 0 to 1097m depth (CSF-A). Total organic carbon ranges from 0.04-0.84%. To date, 300 samples have been solvent-extracted and prepared for plant wax analyses. Long chain n-alkanoic acids and n-alkanes were identified and quantified using GC-MS and GC-FID, respectively. In the samples quantified so far, we find ΣC24-34 n-alkanoic acid concentrations from 0.07-14.16 μg/g of dry sediment and ΣC25-35 n-alkanes from 0.04-4.61 μg/g. Concentrations of C30 n-alkanoic acid range from 0.01-1.92 μg/g of dry sediment and of C33 n-alkane from <0.01-0.65 μg/g. The molecular abundance distributions of both compound-classes were found to be diagnostic of a terrestrial higher plant source. Additionally, the molecular composition of the total lipid extract was analyzed at the Woods Hole Oceanographic Institution using a GC-TOF-MS. Overall, these extracts are dominated by plant-wax compounds and other diagnostic terrestrial molecules (e.g. plant terpenoids and sterols). The results from this effort contribute to a larger mission to reconstruct vegetation and climate change, over the past 20 million years in the core of the monsoon-influenced region, through compound-specific isotope analyses of the plant waxes extracted from these samples.