Engineered Photorespiratory Bypass Pathways Improve Photosynthetic Efficiency and Growth as Temperature Increases
Abstract
In C3 plants grown under ambient [CO2] at 25°C, 23% of the fixed carbon dioxide is lost to photorespiration, the energy expensive metabolic pathway that recycles toxic compounds produced by Rubisco oxygenation reactions. Furthermore, rates of photorespiration increase with rising temperature, as higher temperatures favor increased Rubisco oxygenation. Modelling suggests that the absence of photorespiration could improve gross photosynthesis by 12-55% under projected climate conditions; however, this is difficult to measure empirically, as photorespiration interacts with several metabolic pathways and is an essential process for all C3 plants grown at ambient [O2]. Introduced biochemical bypasses to the native photorespiration pathway hold promise as a strategy to mitigate the impact of temperature on photorespiratory losses. We grew tobacco containing engineered pathways to bypass photorespiration under ambient and elevated temperatures (+5°C) in the field to determine if bypassing photorespiration could mitigate high temperature induced losses in growth and physiology. Our preliminary results show that engineered plants have a higher quantum efficiency under heated conditions than do non-engineered plants, resulting in up to 20% lower yield losses under heated conditions compared to non-engineered plants. These results support the theoretical modelling of temperature impacts on photorespiratory losses, and suggest the bypassing photorespiration could be an important strategy to increase crop yields.
- Publication:
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AGU Fall Meeting Abstracts
- Pub Date:
- December 2017
- Bibcode:
- 2017AGUFM.B53I..05C
- Keywords:
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- 0412 Biogeochemical kinetics and reaction modeling;
- BIOGEOSCIENCES;
- 0414 Biogeochemical cycles;
- processes;
- and modeling;
- BIOGEOSCIENCES;
- 1615 Biogeochemical cycles;
- processes;
- and modeling;
- GLOBAL CHANGE;
- 1631 Land/atmosphere interactions;
- GLOBAL CHANGE