Multi-proxy reconstructions of hydrologic change during the Eocene-Oligocene transition in the North American Interior

The dramatic shift from a 'greenhouse' to an 'icehouse' world that occurred during the Eocene-Oligocene transition (EOT, 34-33.5 Ma) is associated with changes in atmospheric and oceanic circulation patterns, extinction events in both marine and terrestrial ecosystems, and the establishment of a continental-scale ice sheet on Antarctica. Terrestrial records of the EOT, however, show limited consensus regarding the intensity and impact of this transition, complicating our understanding of how terrestrial hydrology responds to climatic change of this magnitude. Stable isotopes of fossil bones and teeth from the White River Group (WRG) in Nebraska have been interpreted to show an 8 °C decrease in mean annual temperatures across the EOT, with an increase in seasonality, however these studies find no evidence for accompanying changes in aridity. Conversely, other studies have inferred increased aridity in the early Oligocene based on changes in the δ18O and δ13C values of mammalian tooth enamel sampled from the same location. Some of this ambiguity is likely to stem from the difficulty in separating the influence of factors such as temperature, water availability and atmospheric circulation patterns on the δ18O composition of ungulate tooth enamel. We present paired leaf wax biomarker and clay hydrogen isotope data from the WRG to produce a new multi-proxy record of hydrological change and accompanying ecosystem shifts through the EOT in the North American interior. Hydrogen isotopes of clay minerals show a negative shift of <15‰ from the late Eocene through early Oligocene, while in contrast leaf wax n-alkanes record a negative shift in excess of 50‰, in tandem with a positive change in average chain length. We infer an increase in aridity from these data, with an associated potential shift in vegetation cover towards more savannah-style plant species as the climate became drier during the transition into the Oligocene. These data provide new constraints on the impact of the EOT in North America, and highlight the potential role of increasing aridity, in addition to declining temperatures, in shaping the hydrology of the region during this interval.