Disturbance of gene expression in primary human hepatocytes by hepatotoxic pyrrolizidine alkaloids: A whole genome transcriptome analysis
Abstract
Structurally different PA highly disturb gene expression in primary human hepatocytes. PA deregulate numerous phase I and II enzymes as well as transport proteins. PA affect cell cycle regulation, cell death and development of cancer. PA interfere with lipid metabolism and bile acid flow.
- Publication:
-
Toxicology in Vitro
- Pub Date:
- October 2015
- DOI:
- 10.1016/j.tiv.2015.06.021
- Bibcode:
- 2015ToxVi..29.1669L
- Keywords:
-
- ABC;
- ATP-binding cassette;
- ACN;
- acetonitrile;
- ADH;
- alcohol dehydrogenase(s);
- ALDH;
- aldehyde dehydrogenase(s);
- b. w.;
- body weight;
- CYP;
- cytochrome P<SUB>450</SUB> monooxygenase(s);
- Em;
- echimidine;
- GST;
- glutathione S-transferase(s);
- Hn;
- heliotrine;
- i. p.;
- intraperitoneal;
- IPA;
- Ingenuity® Pathway Analysis;
- PA;
- pyrrolizidine alkaloid(s);
- PBS;
- phosphate-buffered saline;
- qRT-PCR;
- real-time quantitative PCR;
- Sc;
- senecionine;
- Sk;
- senkirkine;
- SULT;
- sulfotransferase(s);
- UGT;
- UDP-glucuronosyltransferase(s);
- Pyrrolizidine alkaloids;
- Transcriptomics;
- Gene expression;
- Hepatotoxicity