Entner-Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1
Abstract
Phototrophic organisms worldwide produce estimated 10 gigatons of sulfoquinovose (SQ) per year; hence, complete degradation of SQ by bacteria is an important part of the biogeochemical sulfur cycle. Here, we show that Pseudomonas putida SQ1 catabolizes SQ to 3-sulfolactate (SL) in analogy to the Entner-Doudoroff pathway for glucose-6-phosphate, involving five newly discovered reactions, enzymes, and genes, and three newly discovered organosulfur intermediates. The SL can be mineralized by other bacteria, thus closing the sulfur cycle within a bacterial community. The genes for the SQ Entner-Doudoroff pathway can be found in genomes of a wide range of Proteobacteria, which shows that SQ utilization is a widespread and important, but still underrecognized, trait of bacteria in all environments where SQ is produced and degraded.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- August 2015
- DOI:
- 10.1073/pnas.1507049112
- Bibcode:
- 2015PNAS..112E4298F