IL-23-mediated mononuclear phagocyte crosstalk protects mice from Citrobacter rodentium-induced colon immunopathology
Abstract
Gut homeostasis and mucosal immune defense rely on the differential contributions of dendritic cells (DC) and macrophages. Here we show that colonic CX3CR1+ mononuclear phagocytes are critical inducers of the innate response to Citrobacter rodentium infection. Specifically, the absence of IL-23 expression in macrophages or CD11b+ DC results in the impairment of IL-22 production and in acute lethality. Highlighting immunopathology as a death cause, infected animals are rescued by the neutralization of IL-12 or IFNγ. Moreover, mice are also protected when the CD103+ CD11b- DC compartment is rendered deficient for IL-12 production. We show that IL-12 production by colonic CD103+ CD11b- DC is repressed by IL-23. Collectively, in addition to its role in inducing IL-22 production, macrophage-derived or CD103- CD11b+ DC-derived IL-23 is required to negatively control the otherwise deleterious production of IL-12 by CD103+ CD11b- DC. Impairment of this critical mononuclear phagocyte crosstalk results in the generation of IFNγ-producing former TH17 cells and fatal immunopathology.
- Publication:
-
Nature Communications
- Pub Date:
- March 2015
- DOI:
- 10.1038/ncomms7525
- Bibcode:
- 2015NatCo...6.6525A