Microbial community transitions across the deep sediment-basement interface
Abstract
Previous studies of microbial abundance and geochemistry in deep marine sediments indicate a stimulation of microbial activity near the sediment-basement interface; yet, the extent to which microbial communities in bottom sediments and underlying crustal habitats interact is unclear. We conducted tag pyrosequencing on DNA extracted from a spectrum of deep sediment-basement samples to try to identify patterns in microbial community shifts across sediment-basement interfaces, focusing on samples from the subsurface of the Juan de Fuca Ridge flank (IODP Expedition 327). Our results demonstrate that sediment and the basaltic crust harbor microbial communities that are phylogenetically connected, but the eveness is characteristic of the environment. We will discuss the microbial community transitions that occur horizontally along fluid flow pathways and vertically across the sediment basement interface, as well as the possible implications regarding the controls of microbial community composition along deep sediment-basement interfaces in hydrothermal systems. We will also highlight efforts to overcome sample contamination in crustal subsurface samples.
- Publication:
-
AGU Fall Meeting Abstracts
- Pub Date:
- December 2015
- Bibcode:
- 2015AGUFM.B11I0556L
- Keywords:
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- 0414 Biogeochemical cycles;
- processes;
- and modeling;
- BIOGEOSCIENCES;
- 0448 Geomicrobiology;
- BIOGEOSCIENCES;
- 0456 Life in extreme environments;
- BIOGEOSCIENCES;
- 0465 Microbiology: ecology;
- physiology and genomics;
- BIOGEOSCIENCES