Nicotine derived genotoxic effects in human primary parotid gland cells as assessed in vitro by comet assay, cytokinesis-block micronucleus test and chromosome aberrations test
Abstract
Genotoxic effects of nicotine between 1μM and 1mM for 1h were investigated. Experiments were performed in vitro in primary cells of human parotid glands. Acinar character of cell culture was proven by alpha-amylase expression. Increase of DNA migration, micronuclei and chromosome aberrations were determined. No cytotoxic effects or an increase of Caspase-3 were measured.
- Publication:
-
Toxicology in Vitro
- Pub Date:
- August 2014
- DOI:
- Bibcode:
- 2014ToxVi..28..838G
- Keywords:
-
- OTM;
- Olive tail moment;
- CBMN;
- cytokinesis-block micronucleus test;
- MN;
- micronucleus;
- CA;
- chromosome aberration;
- MMS;
- methyl-methane-sulfonate;
- Nicotine;
- Genotoxicity;
- Salivary gland;
- Comet assay;
- Micronucleus;
- Chromosome aberration