Plasmid-based human norovirus reverse genetics system produces reporter-tagged progeny virus containing infectious genomic RNA
Abstract
Human noroviruses are the predominant cause of acute gastroenteritis worldwide, but they remain noncultivatable. A tractable system is needed to understand the host restriction to cultivation. We established a reverse genetics system driven by a mammalian elongation factor-1α promoter without helper virus. This system supports genome replication, particle formation, and particles containing a GFP-marked genomic RNA. RNA from these particles is infectious. The system also produces infectious murine norovirus, confirming its broad applicability to other noroviruses.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- September 2014
- DOI:
- 10.1073/pnas.1415096111
- Bibcode:
- 2014PNAS..111E4043K