Pch2 is a hexameric ring ATPase that remodels the chromosome axis protein Hop1
Abstract
The conserved PCH2 gene in baker's yeast regulates meiotic double-strand break repair outcomes, helps establish a proper meiotic chromosome structure, and is important for the progression of meiotic recombination. Its mouse homolog is required for fertility. However, the molecular mechanism of how PCH2 regulates these diverse functions is not known. In this study, we show that Pch2 is an AAA+ family ATPase (ATPases associated with diverse cellular activities) that oligomerizes into single hexameric rings. In the presence of ATP, Pch2 binds to and remodels Hop1, an important component of the synaptonemal complex, and displaces it from DNA. Based on these and previous observations, we suggest that Pch2 impacts meiotic chromosome organization by directly regulating Hop1 binding to DNA.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- January 2014
- DOI:
- 10.1073/pnas.1310755111
- Bibcode:
- 2014PNAS..111E..44C