Structural basis for the extended CAP-Gly domains of p150glued binding to microtubules and the implication for tubulin dynamics
Abstract
This study presents a direct visualization of the microtubule-p150glued(CAP-Gly) complex by cryo-EM and seeks to describe the molecular mechanism of the control of tubulin dynamics by p150 CAP-Gly. It highlights the neutralization of the acidic tubulin surface by the basic extensions of CAP-Gly, resulting in the activation of tubulin polymerization. In the condition where the lateral association is impeded (i.e., at low temperature), the extended CAP-Gly domain induces tubulin dimers to connect longitudinally. The two directional modes of self-association of tubulin suggest a foundation for its dynamic behavior at the tip of microtubules and its regulation.
- Publication:
-
Proceedings of the National Academy of Science
- Pub Date:
- August 2014
- DOI:
- 10.1073/pnas.1403135111
- Bibcode:
- 2014PNAS..11111347W