Characterization and development of photoactivatable fluorescent proteins for single-molecule-based superresolution imaging
Abstract
Photoactivatable fluorescent proteins (PAFPs) are important probes for superresolution fluorescence microscopy, which allows the spatial organization of proteins in living cells to be probed with sub-diffraction-limit resolution. Here, we compare four properties of PAFPs that are critical for superresolution imaging and report two new PAFPs that exhibit excellent performance in all four properties.
- Publication:
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Proceedings of the National Academy of Science
- Pub Date:
- June 2014
- DOI:
- 10.1073/pnas.1406593111
- Bibcode:
- 2014PNAS..111.8452W